rabbit monoclonal antibody against trpv1  (Cell Signaling Technology Inc)

Journal: BMC Anesthesiology

Article Title: Sevoflurane modulates breast cancer cell survival via modulation of intracellular calcium homeostasis

doi: 10.1186/s12871-020-01139-y

Figure Lengend Snippet: Sevoflurane partially reverses the reduction in cell survival by TRPV1 channel antagonism. Inhibition of Ca 2+ influx through the TRPV1 receptor Ca 2+ channel with the selective antagonist SB-366791 reduces cell survival in all three cell lines. Concomitant use of sevoflurane, but not propofol, partially reversed this effect. Data represents means ± SD from at least 9 repeats of 3 separate experiments and was analyzed by two-way ANOVA followed by Turkey multiple comparison tests. * P < 0.05, **** P < 0.0001

Article Snippet: The membranes were blocked with 5% nonfat dry milk dissolved in PBS with 0.2% TWEEN 20 for 1 h at room temperature and incubated overnight at 4 °C with the primary rabbit monoclonal antibody against TRPV1 (1:1000) (Cell Signaling Technology, Danvers, MA, USA) or primary mouse monoclonal antibody against β-actin (1:3000) (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Inhibition, Comparison

Journal: BMC Anesthesiology

Article Title: Sevoflurane modulates breast cancer cell survival via modulation of intracellular calcium homeostasis

doi: 10.1186/s12871-020-01139-y

Figure Lengend Snippet: Effect of general anesthetics on TRPV1 channel expression. a TRPV1 protein expression in all three cell lines was quantified using the cropping of the Western blot. The β-actin was used as a loading control. Full-length blots are presented in Supplementary Fig. 8. b Treatment with equipotent doses of sevoflurane (2%) and propofol (2 μM) for 6 h did not affect TRPV1 protein expression in any of the three cell lines. All values shown are means ± SD of triplicate repeats from three separate experiments and were analyzed by one-way ANOVA

Article Snippet: The membranes were blocked with 5% nonfat dry milk dissolved in PBS with 0.2% TWEEN 20 for 1 h at room temperature and incubated overnight at 4 °C with the primary rabbit monoclonal antibody against TRPV1 (1:1000) (Cell Signaling Technology, Danvers, MA, USA) or primary mouse monoclonal antibody against β-actin (1:3000) (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Western Blot, Control